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Mylne publications

On this page you will find links to articles written for Popular Media and an interview (below), six recent highlights (top, right) as well as a complete list of publications. Many are linked to the PubMed or the publishing house via the DOI. For difficult to access articles, a link to a PDF is included instead.

For an up-to-date list of publications and their citations go to Josh Mylne's ResearcherID page.


ACS Chem. Biol., May 2019

Franke et al. "An ancient peptide family buried within vicilin precursors" Art by Scot Nicholls, Domokun Design

Nat. Prod. Rep., February 2018

Franke et al. "Buried treasure: biosynthesis, structures and applications of cyclic peptides hidden in seed storage albumins" Art by Johan Rosengren

J. Biol. Chem., 28 July 2017

Franke et al. "Two proteins for the price of one: structural studies of the dual-destiny Preproalbumin with Sunflower Trypsin Inhibitor-1" Image by Roman Eder, Art by Johan Rosengren

Plant Cell, March 2017

James et al. "Evidence for ancient origins of Bowman-Birk Inhibitors from Selaginella moellendorffii" Artwork by Scot Nicholls, Domokun Design

Org. Biomol. Chem., 28 May 2015

Gandy et al. "An interactive database to explore herbicide physicochemical properties" Artwork by Maxime Corral

Chem. Biol., 21 May 2015

Bernath-Levin et al. "Peptide macrocyclization by a bifunctional endoprotease" Artwork by Scot Nicholls, Domokun Design

Plant Cell, March 2014

Elliott et al. "Evolutionary origins of a bioactive peptide buried within preproalbumin" Artwork by Scot Nicholls, Domokun Design

Aust. Sci., May 2014

Mylne "Genes that cuddle when it's cold". Artwork by Aust. Sci.

Funct. Plant Biol., 2014

Mylne, Hara-Nishimura & Rosengren "Seed storage albumins: biosynthesis, trafficking and structures". Artwork by J. Mylne

DNA in pieces

Chem. Comm., Feb. 2018
"Developing ciprofloxacin analogues against plant DNA gyrase: a novel herbicide mode of action" PubMed
Taking work we published with UK scientists in JBC, 2016 to the next stage, we found analogs of ciprofloxacin with increased herbicidal activity and diminished antibacterial activity based on their activity against gyrase.

Herbicide targets

Plant Journal, June 2018
"Targeting plant DIHYDROFOLATE REDUCTASE with antifolates and mechanisms for genetic resistance" PubMed
The observation we reported in Sci. Rep., 2017 that most antimalarials are herbicidal, spurred our desire to know more about DHFR in plants as it's a major target of antimalarials, but not herbicides.

Buried treasure

Mol. Biol. Evol., June 2017
"Stepwise evolution of a buried inhibitor peptide over 45 million years" PubMed
Building on work in Nature Chem. Biol., 2011 and Plant Cell, 2014 we use 110 transcriptomes and phylogenies to document the stepwise evolution of a peptide family inside precursors for the usually dull seed storage albumin.

Malaria vs weeds

Angewandte Chem., Aug 2017
"Exploiting the evolutionary relationship between malarial parasites and plants to develop new herbicides." PubMed
Trawling a library of antimalarial leads we struck one that was a good herbicide, performed structure-activity analysis and tested it with agrochemical company BASF.

Cut & glue

eLife, March 2018
"Structural basis of ribosomal peptide macrocyclization in plants" PubMed
Building on work in Chem. Biol., 2015 we reveal the structure of a sunflower seed AEP and shows what treatment enables it to cyclize. We were helped by a tetrahedral intermediate seen in its active site.

BBIs in spikemoss

Plant Cell, March 2017
"Evidence for ancient origins of Bowman-Birk inhibitors from Selaginella moellendorffii" PubMed
Proteins doppelgängers end up long lost cousins! Finding Bowman-Birk Inhibitors in spikemoss changed the understanding of ancestry of these proteins previously found only in cereals and legumes.

Mylne research publications

Following is a complete list of publications in reverse chronological order.

Fisher, Payne, Rosengren, Mylne (2019) An orbitide from Ratibida columnifera seed containing 16 amino acid residues. J. Nat. Prod. online. PubMed, DOI.

Pullella, Wdowiak, Sykes , Lucatoni, Sukhoverkov, Zulfiqar , Sobolev, West, Mylne, Avery, Piggott (2019) Total synthesis of the antimalarial ascidian natural product albopunctatone. Org. Lett. online 9 July 2019. PubMed, DOI.

Fisher, Zhang, Berkowitz, Whelan, Mylne (2019) Novel cyclic peptides in seeds of Annona muricata are ribosomally synthesised. bioRxiv Posted May 25, 2019. DOI.

Pouvreau, Fenske, Ivanova, Murcha, Mylne (2019) An interstitial peptide is readily processed from within seed proteins. Plant Science 285: 175-183. PubMed, DOI.

Zhang, Payne, Pouvreau, Schaefer, Fisher, Taylor, Berkowitz, Whelan, Rosengren, Mylne (2019) An ancient peptide family buried within vicilin precursors. ACS Chemical Biology 14: 979-993. PubMed, DOI.

Behsaz, Mohimani, Gurevich, Prjibelski, Fisher, Smarr, Dorrestein, Mylne, Pevzner (2019) De novo peptide sequencing reveals a vast cyclopeptidome in human gut and other environments. bioRxiv Posted January 16, 2019. DOI.

James, Haywood, Leroux, Ignasiak, Elliott, Schmidberger, Fisher, Nonis, Fenske, Bond, Mylne (2019) The macrocyclizing protease butelase 1 remains auto-catalytic and reveals the structural basis for ligase activity. Plant Journal 98: 988-999. PubMed, DOI.

Corral, Haywood, Stehl, Stubbs, Murcha, Mylne (2018) Targeting plant DIHYDROFOLATE REDUCTASE with antifolates and mechanisms for genetic resistance. Plant Journal 95: 727-742. PubMed, Publisher.

Fisher, Zhang, Taylor, Howard, Berkowitz, Debowski, Behsaz, Whelan, Pevzner, Mylne (2018) A family of small, cyclic peptides buried in preproalbumin since the Eocene epoch Plant Direct 2: e00042. PubMed, Publisher.

Haywood, Schmidberger, James, Nonis, Sukhoverkov, Elias, Bond, Mylne (2018) Structural basis of ribosomal peptide macrocyclization in plants. eLife 7: e32955. PubMed, Publisher.

Corral, Leroux, Tresch, Newton, Stubbs*, Mylne* (2018) A herbicide structure-activity analysis of the antimalarial lead compound MMV007978 against Arabidopsis thaliana. Pest Manag. Sci. 77: 1558-1563. PubMed, Publisher.

Franke, Mylne, Rosengren (2018) Buried treasure: biosynthesis, structures and applications of cyclic peptides hidden in seed storage albumins. Nat. Prod. Rep. 35: 137-146. PubMed, Publisher.

Wallace, Waraich, Debowski, Corral, Maxwell, Mylne*, Stubbs* (2018) Developing ciprofloxacin analogues against plant DNA gyrase: A novel herbicide mode of action. Chem. Commun. 54: 1869-1872. PubMed, Publisher.

James, Haywood, Mylne (2018) Macrocyclization by asparaginyl endopeptidases. New Phytologist 218: 923-928. PubMed, Publisher.

Corral, Leroux, Tresch, Newton, Stubbs*, Mylne* (2017) Exploiting the evolutionary relationship between malarial parasites and plants to develop new herbicides. Angew. Chem. Int. Ed. Engl. 56: 9881-9885. PubMed, Publisher.

Franke, James, Mobli, Colgrave, Mylne, Rosengren (2017) Two proteins for the price of one: structural studies of the dual-destiny Preproalbumin with Sunflower Trypsin Inhibitor-1. J. Biol. Chem. 292: 12398-12411. PubMed, Publisher.

Corral, Leroux, Stubbs, Mylne (2017) Herbicidal properties of antimalarial drugs Scientific Reports 7: 45871. PubMed, Publisher.

Jayasena, Fisher, Panero, Secco, Bernath-Levin, Berkowitz, Taylor, Schilling, Whelan, Mylne (2017) Stepwise evolution of a buried inhibitor peptide over 45 million years. Molecular Biology and Evolution 34: 1505-1516. PubMed, Publisher.

James, Jayasena, Zhang, Berkowitz, Secco, Knott, Whelan, Bond, Mylne (2017) Evidence for ancient origins of Bowman-Birk inhibitors from Selaginella moellendorffii. Plant Cell 29: 461-473. PubMed, Publisher.

Grage, Sani, Cheneval, Henriques, Schalck, Heinzmann, Mylne, Mykhailiuk, Afonin, Komarov, Separovic, Craik, Ulrich (2017) Orientation and location of the cyclotide kalata B1 in phospholipid bilayers revealed by solid-state NMR. Biophysical Journal 112: 630-642. PubMed, Publisher.

Elliott, Franke, Armstrong, Craik, Mylne*, Rosengren* (2017) Natural structural diversity within a conserved cyclic peptide scaffold. Amino Acids 49: 103-116. PubMed, Publisher.

Franke, Jayasena, Fisher, Swedberg, Taylor, Mylne, Rosengren (2016) Diverse cyclic seed peptides in the Mexican zinnia (Zinnia haageana). Biopolymers 106: 806-817. PubMed, Publisher.

Franke, Colgrave, Mylne, Rosengren (2016) Mature forms of the major seed storage albumins in sunflower: a mass spectrometric approach. Journal of Proteomics 147: 177-186. PubMed, Publisher.

Evans-Roberts, Mitchenall, Wall, Leroux, Mylne, Maxwell (2016) DNA gyrase is the target for the quinolone drug ciprofloxacin in Arabidopsis thaliana. J. Biol. Chem. 291: 3136-3144. PubMed, Publisher.

Jayasena, Franke, Rosengren, Mylne (2016) A tripartite approach identifies the major sunflower seed albumins. Theoretical and Applied Genetics 129: 613-629. PubMed, Publisher.

Bernath-Levin, Nelson, Elliott, Jayasena, Millar, Craik, Mylne (2015) Peptide macrocyclization by a bifunctional endoprotease. Chemistry & Biology 22: 571–582. PubMed, Publisher.

Gandy, Corral, Mylne*, Stubbs* (2015) An interactive database to explore herbicide physicochemical properties. Organic & Biomolecular Chemistry 13: 5586-5590. (*co-corresponding) PubMed, Publisher.

Mahatmanto, Mylne, Poth, Swedberg, Kaas, Schaefer, Craik (2015) The evolution of Momordica cyclic peptides. Molecular Biology and Evolution 32: 392-405. PubMed, Publisher.

Jayasena, Secco, Berkowitz, Bernath-Levin, Whelan, Mylne (2014) Next generation sequencing and de novo transcriptomics to study gene evolution. Plant Methods 10: 34. PubMed, Publisher.

Elliott, Delay, Liu, Phua, Rosengren, Benfield, Panero, Colgrave, Jayasena, Dunse, Anderson, Schilling, Ortiz-Barrientos, Craik, Mylne (2014) Evolutionary origins of a bioactive peptide buried within preproalbumin. Plant Cell 26: 981-995. PubMed, Publisher.

Mylne, Hara-Nishimura, Rosengren (2014) Seed storage albumins: biosynthesis, trafficking and structures (Goldacre Review). Functional Plant Biology 41: 671-677. DOI, Publisher.

Mahatmanto, Poth, Mylne, Craik (2014) A comparative study of extraction methods reveals preferred solvents for cystine-knot peptide isolation from Momordica cochinchinensis seeds. Fitoterapia 95: 22-33. PubMed, Publisher.

Rosa*, De Lucia*, Mylne*, Zhu*, Ohmido, Pendle, Kato, Shaw, Dean (2013) Physical clustering of FLC alleles during Polycomb-mediated epigenetic silencing in vernalization. Genes & Development 27: 1845-1850. (*equal) PubMed, Publisher.

King, Chanson, McCallum, Ohme-Takagi, Byriel, Hill, Martin, Mylne (2013) The Arabidopsis B3 domain protein VERNALIZATION1 is involved in processes essential for development with structural and mutational studies revealing its DNA binding surface. J. Biol. Chem. 288: 3198–3207. PubMed, Publisher.

Mylne, Chan, Chanson, Daly, Schaefer, Bailey, Nguyencong, Cascales, Craik (2012) Cyclic peptides arising by evolutionary parallelism via asparaginyl-endopeptidase mediated biosynthesis. Plant Cell 24: 2765-2778. PubMed, Publisher.

Poth, Mylne, Grassl, Lyons, Millar, Colgrave, Craik (2012) Cyclotides associate with leaf vasculature and are the products of a novel precursor in Petunia (Solanaceae). J. Biol. Chem. 287: 27033-27046. PubMed, Publisher.

Craik, Henriques, Mylne, Wang (2012) Cyclotide isolation, characterization and bioassay. in Natural product biosynthesis by microorganisms and plants (3rd edition) Methods in Enzymology 516: 37-62. PubMed, Publisher.

Craik, Swedberg, Mylne, Cemazar (2012) Cyclotides as a basis for drug design. Expert Opinion on Drug Discovery 7:179-194. PubMed, Publisher

Mylne, Mas, Hill (2012) NMR assignment and secondary structure of the C-terminal DNA binding domain of Arabidopsis thaliana VERNALIZATION1. Biomolecular NMR Assignments 6: 5-8. PubMed, Publisher.

Mohimani, Liu, Mylne, Poth, Colgrave, Tran, Selsted, Dorrestein, Pevzner (2011) Cycloquest: Identification of cyclopeptides via database search of their mass spectra against genome databases. Journal of Proteome Research 10: 4505-4512. PubMed, Publisher.

Mylne & Wigge (2011) Florigen takes two to tango. Nature Chemical Biology 7:665-666. PubMed, Publisher.

Elliott, Craik, Mylne (2011) Sunflower trypsin inhibitor-1: chemical v. biological synthesis. Proceedings of the 22nd American Peptide Symposium, ed. Michal Lebl, Prompt Scientific Publishing, San Diego, CA, pp86-87. PDF.

Mylne, Colgrave, Daly, Chanson, McCallum, Jones, Elliott, Craik (2011) Albumins and their processing machinery are hijacked for cyclic peptides in sunflower. Nature Chemical Biology 7: 257-259. PubMed, Publisher.

Box, Coustham, Dean, Mylne (2011) Protocol: A simple phenol-based method for 96-well extraction of high quality RNA from Arabidopsis. Plant Methods 7: 7. PubMed, Publisher.

Mylne, Wang, van der Weerden, Craik (2010). Cyclotides are a component of the innate defence of Oldenlandia affinis. Biopolymers (Pept. Sci.) 94: 635-646. PubMed, Publisher.

Qin, McCallum, Kaas, Saska, Craik, Mylne (2010). Identification of candidates for cyclotide biosynthesis and cyclization by expressed sequence tag analysis of Oldenlandia affinis. BMC Genomics 11: 111. PubMed, Publisher.

Craik, Mylne, Daly (2010). Cyclotides: macrocyclic peptides with applications in drug design and agriculture. Cell. Mol. Life Sci. 67: 9-16. PubMed, Publisher.

Trabi, Mylne, Sando, Craik (2009). Circular proteins from Melicytus (Violaceae) refine the conserved protein and gene architecture of cyclotides. Organic & Biomolecular Chemistry. 7: 2378 – 2388. PubMed, Publisher.

King, Hill, Martin, Mylne (2009). Expression, purification and preliminary X-ray diffraction studies of VERNALIZATION1208-341 from Arabidopsis thaliana. Acta Crystallogr. F 65: 291-294. PubMed, Publisher.

Pien, Fleury, Mylne, Crevillen, Inzé, Avramova, Dean, Grossniklaus (2008). ARABIDOPSIS TRITHORAX1 dynamically regulates FLOWERING LOCUS C activation via histone 3 lysine 4 trimethylation. Plant Cell 20: 580-588. PubMed, Publisher.

Mylne & Craik (2008). 15N cyclotides by whole plant labeling. Biopolymers (Pept. Sci.) 90: 575-580. PubMed, Publisher.

Herrmann, Burman, Mylne, Karlsson, Gullbo, Craik, Clark, Göransson (2008). The alpine violet, Viola biflora, is a rich source of novel cyclotides with potent cytotoxicity. Phytochemistry 69: 939-952. PubMed, Publisher.

Greb*, Mylne*, Crevillen, Geraldo, An, Gendall, Dean (2007). The PHD finger protein VRN5 functions in the epigenetic silencing of Arabidopsis FLC. Current Biology 17: 73-78. (*equal). PubMed, Publisher.

Mulvenna, Mylne, Bharathi, Burton, Shirley, Fincher, Anderson, Craik (2006). Discovery of cyclotide-like protein sequences in graminaceous crop plants: ancestral precursors of circular proteins? Plant Cell 18: 2134-2144. PubMed, Publisher.

Mylne, Barrett, Tessadori, Mesnage, Johnson, Bernatavichute, Jacobsen, Fransz, Dean (2006). LHP1, the Arabidopsis homologue of HETEROCHROMATIN PROTEIN1 is required for epigenetic silencing of FLC. Proc. Natl Acad. Sci. U.S.A. 103: 5012-5017. PubMed, Publisher.

Petsch, Mylne, Botella (2005). Cosuppression of eukaryotic release factor 1-1 in Arabidopsis affects cell elongation and radial cell division. Plant Physiology 139: 115-126. PubMed, Publisher.

Mylne, Greb, Lister and Dean (2004). Epigenetic Regulation in the Control of Flowering. in Epigenetics, eds. Stillman & Stewart (CSHL Press, Cold Spring Harbor, New York), Vol. LXIX, pp. 457–464. PubMed, PDF.

Bastow, Mylne, Lister, Lippman, Martienssen, Dean (2004). Vernalization requires epigenetic silencing of FLC by histone methylation. Nature 427: 164-167. PubMed, Publisher.

Boss, Bastow, Mylne, Dean (2004). Multiple pathways in the decision to flower: enabling, promoting, and resetting. Plant Cell 16: S18-31. PubMed, Publisher.

Levy, Mesnage, Mylne, Gendall, Dean (2002). Multiple roles of Arabidopsis VRN1 in vernalization and flowering time control. Science 297: 243-246. PubMed, Publisher.

Mylne, Botella (1998). Binary vectors for sense and antisense expression of Arabidopsis ESTs. Plant Molecular Biology Reporter 16: 257-262. Publisher.

Mylne, Mason, Botella (1998). Rapid isolation of high-quality RNA from symbiotic dinoflagellates. Phycologia 37: 307-309. Publisher.