We are an established and growing group of people interested in plant molecular biology, genetics, biochemistry and chemical biology. Our lab was established at UWA in January 2013 and in mid-2021 move to Curtin University. Our current and recent substantive funding is:
- LP200301658 (Mylne, Stubbs, Williams, Lee) "Rethinking and revitalising herbicides to counter resistance"
- Nexgen-UWA Herbicide Partnership (Mylne, Stubbs & Haywood) with Nexgen Plants
- DP190101048 (Mylne, Stubbs, Haywood, Maxwell) "Putting new herbicide targets on the table"
- DP190102058 (Mylne, Rosengren, Hara-Nishimura, van der Hoorn) "Buried treasure: bioactive plant seed proteins evolving inside hosts"
- For all funding see Projects on UWA respository
RNA biology, genomics and epigenomics, plant genetics, organelle biogenesis and computational biology.
Structural biology, synthetic biology, chemical biology, drug design, metabolomics, bionano and enzymology.
Catalysis, molecular electronics, molecular materials, theoretical and computational chemistry.
Built to high specifications for containment up to PC3, Building 304 is home to Curtin's Centre for Crop and Disease Management (CCDM). The science there straddles three disciplines:
Top one per cent in the world. Curtin is ranked in the top one per cent of universities worldwide in the highly regarded Academic Ranking of World Universities (ARWU) 2020.Academic Ranking of World Universities
Western Australia’s most preferred university. Around half of Western Australia’s undergraduate applicants nominate Curtin University as their first preference. In 2019 it was 53.3 per cent.Curtin University
Research on the rise. 95 per cent of Curtin's research output was rated at or above world standard in the 2018 Excellence in Research for Australia results.ARC Research Excellence
Mario Bani, M.Phil. student
Mario earned his Masters in peptide biochem from the Institute for Molecular Bioscience at the University of Queensland working on recombinant production of spider peptide toxins. He is using Arabidopsis genetics and recombinant protein biochemistry to validate new herbicide targets.
Joel Haywood, Research Fellow
Joel did his undergraduate and a 3-year industry stint in the UK before a PhD on leukocyte receptor structure at the Chinese Academy of Science in Beijing. He is working on a range of protein projects where we seek to bring a structural understanding of biosynthesis and bioactivities.
Karsoon Ng, Hons student
Karsoon did his undergrad at Curtin University and has come to work on novel herbicidal compounds that target HMGR working close with Joel Haywood. Karoon is using large scale docking with new protein structures combined with cheminformatics to find potential new herbicides.
Maike Bollen, Research Associate
Before joining the lab, Maike worked for a decade at UWA in CMCA for Metabolomics Australia mostly in the area of GC-MS. Now she's the gatekeeper of the lab's Thermo OrbiTrap Exploris 120 being used to follow fungicide biotransformation, starting with tebuconazole.
Jordan Campbell, Hons student
Jordan did his undergrad at Curtin University is working with our new OrbiTrap to examine the biotransformation of flutriafol specifically in planta. He's working collaboratively in chemical aspects with Dr Hendra Gunosewoyo with synthetic versions to confirm some of the compounds he is finding in planta.
Michael Wallace, PhD student
Michael did his BSc in chemistry at UWA and is jointly supervised with Assoc. Prof. Keith Stubbs, in whose lab he is working with a potentially new class of herbicide. Using synthetic organic chemistry, Michael is creating analogs to find ones that make better herbicides by testing the analogs in Arabidopsis and other systems.
Karen Breese, Post-doc
Dr Breese is an organic chemist who did her PhD at ANU in Canberra and then worked for some years in industry at Epichem here in Perth before joining the herbicide team at UWA. Karen is based in the lab of Assoc. Prof. Keith Stubbs, in whose lab she is working on organic chemistry synthesis of new herbicides.
Joshua Mylne, lab head
Josh has a PhD (2002) in plant genetic engineering from UQ, did a 4-year post doc in Arabidopsis molecular genetics and epigenetics at the John Innes Centre (UK) before a 7-year fellowship stint with peptide chemists at the IMB, UQ. He currently yearns to get into the lab for more than the occasional RNA extraction.
Sam Nonis, 2021 PhD (UWA)
"Having spent a year experimenting with the directed evolution of proteins at the Agency for Science, Technology and Research (A*STAR) in Singapore, I became interested in how protein structure relates to function. I pursued that interest while learning to become a structural biologist in Josh’s research group, where I was a PhD student from 2017 to 2021. Josh is keenly aware of the important role that he plays as a group leader. He aims for his lab members to become and be successful scientists with promising careers. One way that Josh does this is by ensuring that we learn to be independent researchers. For example, Josh makes sure that every research group member learns to write their own stories for their research papers. He encourages and facilitates this process by running frequent writing retreats, where he imparts fundamental skills for manuscript writing. Josh also encouraged me to take the lead on a collaboration, where I learnt important soft skills often overlooked in research. He also made it a point to increase his presence when the going got tough. I completed a successful PhD thanks in large part to Josh’s careful guidance." (2 June 2021)
Dr Maxime Corral, 2018 PhD (UWA)
"I moved to Perth in 2014 to start a PhD in the Mylne Lab after completing my Master’s Degree in Plant Biotechnology from the University of Otago (New Zealand). Prior to arriving to Australia, I was granted a highly competitive PhD scholarship for international students (UWA) with support and help from Josh. My research projects looked at unconventional herbicidal compounds and their modes of action in Arabidopsis. From my PhD experience, I have learnt a large array of molecular, biochemical and genetics techniques. Josh made sure to equip me with the skills necessary for publishing high quality research articles which, I have no doubt, helped me obtain a post-doctoral fellowship at CSIRO in 2018. Hailing from France AND as a member of the LGBTIQA+ community, I was very grateful of being part of a lab that promotes inclusion, diversity and respect. Josh’s lab was affiliated to the Plant Energy Biology centre (ARC) and as such, I was extremely fortunate to have access to further laboratory equipment, growth facilities and peers’ expertise which greatly supported the success of my PhD at UWA." (27 May 2021)
Dr Amy James, 2018 PhD (UWA)
"I joined Josh’s lab as a PhD candidate after completing my Undergraduate and Masters degree at University of Victoria, BC, Canada. I submitted my PhD as a series of publications which included two published first author publications, one first author publication ready for submission, one co-authored published manuscript and one co-authored publication ready for submission. During my PhD, I developed numerous techniques in molecular biology and biochemistry. Along with Josh’s background in plant genetics and molecular biology, my co-supervisor Prof. Charlie Bond, with whom Josh maintains an active collaborative relationship, has exceptional knowledge and expertise in structural biology. Josh’s supervisory philosophy is to prepare his students for the next stage in their career by providing a well rounded and fulfilling experience. Beyond scientific ability, Josh encourages students to develop other skills important to a successful scientific career. For example, I had the opportunity to take part in all aspects of producing a published research article including writing of the manuscript, generating figures and writing the response to reviewers. He offers several workshops to assist in these skills including a photoshop/illustrator course and a bi-annual writing retreat." (9 Feb 2018)
Dr Alysha Elliott, 2013 PhD (UQ)
"Starting a graduate program in 2009 as Associate Professor Josh Mylne’s first primary PhD student was an exciting, challenging and rewarding time for us both. It was a great position to be in with a young and enthusiastic post doctoral supervisor who was keen to be by my side in the laboratory when needed as well as giving me the independence to find my own grounding and niche as a scientist. My thesis “A New Class of Daisy Seed Peptides” describes the discovery of a new and structurally diverse class of seed peptides. The majority of this work was [recently accepted] for publication in Plant Cell and other elements are currently being continued in the lab. I learnt a vast number of new techniques, as this project was multidisciplinary based on Josh’s plant molecular biology expertise, my own prior skill set in peptide chemistry and, via a strong collaboration with Dr. Johan Rosengren (NMR expert), structural biology. During my candidature, with the passionate support of Josh, I had the opportunity to attend international conferences, where I won student bursaries and prizes for my work, visited international collaborators to collect samples on a student travel award and competed in the GlaxoSmithKline student awards. My time in the Mylne lab was highly successful and enjoyable thanks to a great group, of not only intelligent and enthusiastic but friendly and supportive young people." (21 Oct 2013)
Christina Delay, 2011 Honours (UQ), First Class, Dean's Commendation
"For my honours project, I set out to investigate how and when cyclic peptides first arose albumin genes in the daisy family. This project gave me the opportunity to use a range of different experimental techniques, including molecular biology, plant biology and protein chemistry. I received First Class Honours with a Dean's Commendation for High Achievement and my results will be included in a forthcoming publication [see here]. Josh is extremely passionate about his work and his enthusiasm certainly rubs off onto his students. The lab has a great dynamic, with a good mix of students and RAs who are always able to offer advice when needed. Additionally, the lab has a number of excellent collaborators. Next year, I will be starting a Ph.D. in plant sciences under the supervision of Assoc. Professor Michael Djordjevic at the Australian National University in Canberra." (3 Dec 2011)
Zaiyang Phua, 2010 Masters (UQ), Dean's Commendation
"I did my Masters in Molecular Biology in 2010 with Josh at the IMB on the genetic evolution of seed peptides and received a grade of straight sevens [7 is the highest mark possible] as well as the Dean's Commendation for High Achievement. It was a tremendously rewarding experience working under Josh and I have learned many invaluable laboratory techniques and skills which I will hold on to for my scientific career. I really enjoyed my time in the lab and Josh's dedication and passion towards his work will always be an inspiration for me. I now work at the Genome Institute of Singapore as a research officer performing next-generation sequencing." (17 Dec 2011)
Dr Kirill Sukhoverkov, 2021 PhD (UWA)
"After working for a couple of years in the field of enzyme chemistry my research interest drifted towards more into biology and I decided to find a biology focused PhD where my chemistry skills still could be relevant. That’s why in 2017 I decided to join Joshua Mylne lab. Being educated in Russia, where the university system is quite different from Australia I had little understanding on how to write things like personal statement, CV and scholarship application. Therefore, I strongly doubted that I could get an offer from UWA. However, Josh understood my challenge and spent a lot of time and effort to help me, a complete stranger to him at that moment, to secure the highly competitive International Postgraduate Research Scholarship. During the four years in the Josh’s lab I learnt more than just a bunch of lab techniques and fancy Illustrator tricks. Most importantly I learnt how to grow idea into a project, and how to make a research project into a publication. So, if you are looking for PhD lab that will make you not a lab robot, but researcher then definitely choose Josh Mylne lab." (11 September 2022)
Dr Jingjing Zhang, 2019 PhD (UWA)
"After I received my Masters degree in biophysics in University of Chinese Academy of Science, I joined the Mylne Lab in 2015 as a UWA PhD student. Thanks for Josh’s help, I was awarded the International Postgraduate Research Scholarship (IPRS) which was very competitive. During the next 4 years in Mylne Lab I focused on revealing the evolutionary origin of an ancient peptide family in seed vicilin and investigating the new herbicidal modes of action. Josh is perhaps the most passionate researcher you can ever find, and a very experienced supervisor as well. He’s always ready to help, and his insights in plant biology can always guide you through. During my PhD I learned a lot about molecular biology, peptide chemistry, bioinformatics, and structural biology, and got a bunch of co-publications including one first-author high quality paper in ACS Chemical Biology. Besides, I will always miss the numerous workshops that Josh hold, in which I have learned very useful manuscript writing skills, as well as the happy beer times afterwards!" (1 June 2021)
Thaveshini Chetty, 2019 undergrad volunteer (UWA)
"I joined Mylne Lab in 2019 as a UWA undergraduate student. Josh proposed a short project rediscovering Evolidine, a cyclic peptide first discovered in Australia. During this exciting project Josh was always very supportive and enthusiastic, he imparted his knowledge generously and patiently. He makes time to engage with all his students and fosters positive growth within his team, I felt lucky to be a part of his lab. He had a huge positive impact on my post-graduate goals and through my experience and research with Josh I gained confidence in my capacity to research and perform molecular biology techniques. Even better, I gained a publication before I had started my Honours [see here]. He is the dream mentor, and the Obi-Wan Kenobi of cyclic peptides." (10 Oct 2020)
Dr Achala Jayasena, 2016 PhD (UWA)
"I completed my Bachelor’s degree in Agriculture from Sri Lanka and gained a Masters of Biology from Adelphi University, New York studying Arabidopsis genetics. With a desire to expand my skill set, I joined what would become the Mylne lab (while Josh was hosted by the Craik lab at IMB, UQ) as a PhD candidate in 2012. I got to be the first student in the official 'Mylne lab' at UWA where I continued working on my project studying the evolution of the dual-fate protein PawS1. My PhD journey (2012-2016) was an extremely successful and interesting one. I developed skills in de novo transcriptomics and proteomics in addition to strengthening my skills in molecular biology. I graduated in 2016 authoring six manuscripts in respected journals and I have a few more on the way. I was lucky enough to submit my PhD thesis as a series of publications. Despite being a small lab, we were well connected with big labs from whom we sought expertise in next generation sequencing, proteomics and daisy evolution. The lab always had a very friendly working environment and it was a pleasant surprise to see our lab head working in the lab along with us most of the time. Josh was a very supportive supervisor with a positive mindset, who helped me with all the steps in my candidature from the scholarship application to the thesis submission. I enjoyed my time in the Mylne lab and thank Josh, my lab colleagues and our collaborators for their wonderful support." (2 Nov 2016)
Joseph O'Neill, 2011-2012 Summer, IMB Summer Scholar
"I was lucky enough to receive an IMB Summer Scholarship for an internship with Assoc. Prof. Mylne. I worked on a project that built on the effect antimalarial drugs have on plants. Specifically, I sterilised and sowed mutated Arabidopsis seed onto antimalarial-drug-containing plates of media, aiming to find a drug-resistant line. It is hoped that, given the shared evolutionary history between malaria parasites and photosynthetic plants, Assoc. Prof. Mylne's work in this area will help elucidate the mode of action of antimalarial drugs in parasites. Being in charge of my own project, albeit with expert guidance, I was able to learn and develop new skills that I was yet to do in my undergraduate education. I learned much about drug design and action, as well as practical skills like sterile technique, liquid and gas seed sterilisation, plant husbandry, making media, following scientific methods, researching techniques, and keeping a lab book. All of which will be useful for my science career. During my time I was supervised by the competent and engaged members of Assoc. Prof. Mylne's and Prof. Craik's group, and as my first experience of the research world my time in Assoc. Prof. Mylne's lab offered me invaluable experience and knowledge. As I am graduating from my B.Sc. in Biochemistry and Molecular Biology at UQ in June, I feel I have made connections at the IMB that will serve me in my postgraduate education and my future career. I had a great summer and thoroughly enjoyed my time at the IMB." (13 Feb 2012)
Kate Martinac, 2011 Honours (UQ), First Class
"I recently completed my honours in B. Biotechnology on in planta peptide processing at the IMB and received First Class Honours. It was a privilege to have Josh as my supervisor and to work with such an intelligent team of researchers in state-of-the-art facilities. I thoroughly enjoyed my time in the lab and found the experience both challenging and rewarding. I now work at QUT bluebox, assisting in the commercialisation of research outcomes." (30 Nov 2011)
Lab Alumni and News
Below are some details of the destiny of all the lab's alumni. Apologies if some of the links are dead.
Mark Fisher: After plans to move back to the UK were derailed by COVID-19, Dr Fisher (2015-2019) landed a 3-year post-doc working on RNAi-mediated plant protection with Lars Kamphius at CCDM. [2021, December]
Kirill Sukhoverkov: Kirill (2017-2021) did his PhD viva the day after landing in Norwich to start a 5 year post-doc contract on bacterial chromosome segregation with Tung Le at The John Innes Centre. See this Tweet. [2021, July]
Jingjing Zhang: More news from lab alumna, Dr Jinging Zhang, who has made the most of sudden border closures by landing her first post-doc, a 3-year position at Shenzhen hospital to research plant metabolites plus some human medicinal work too. See this Tweet. [2020, June]
Jingjing Zhang: While waiting for her thesis to be reviewed, PhD student (2015-2019) Jingjing took up a job with the Department of Primary Industries and Regional Development here in Perth, see this 2020 news release on a new footrot test she was involved in. [2020, January]
Julie Leroux: Working as an RA in our lab on herbicides (2013-2016) has inspired Julie to go back to school and this month she started a PhD with Prof. Barry Pogson at ANU where she works biosynthesis pathway of the apocarotenoid signal, ACS1, in Arabidopsis. [2018, May]
Amy James: While finishing off her thesis corrections, PhD student (2014-2017) Amy got the word that she would be off to Bristol University's School of Biological Sciences to work with Gary Foster on cassava brown streak virus, starting in June 2018. [2018, March]
Achala Jayasena: After an exceptionally productive PhD (2013-2016) Achala took a break to add to her family and this month starts a short contract as a technician at CSIRO in nearby Floreat. [2018, March]
Maxime Corral: Just before he handed his thesis in for examination, PhD student (2014-2017) Max was offered a 3-year post-doctoral fellowship with CSIRO to work with Dr Jonathan Anderson in Floreat, Perth where he will develop novel technologies to control crop diseases. His job starts January 2018. [2017, December]
Bastian Franke: Not long after handing in his thesis, joint PhD student (2014-2017) Bastian was offered a 3-year post-doctoral position in NMR structural biology with Stephan Grzesiek at the Biozentrum at the University of Basel in Switzerland. He started his new job in May 2017. [2017, April]
Benjamin Pouvreau: Just before he handed his thesis in, PhD student (2013-2016) Benjamin was offered a 3-year post-doctoral fellowship with CSIRO in Canberra where he will work with a multi-disciplinary team of scientists examining canola oil biosynthesis. He took up the position in late July. [2016, July]
Kalia Bernath-Levin: After ~2 years in our lab, Kalia secured a 5-year, senior post-doctoral position with ARC Laureate Fellow Prof. Ian Small in the ARC Centre of Excellence in Plant Energy Biology where she is working in the area of synthetic biology and PPR proteins. [2015, March]
Alysha Elliott: Straight from her PhD (2009-2013) Alysha started a position as a post-doctoral project manager with NHMRC Australia Fellow Professor Matt Cooper optimising an antibacterial screening pipeline for the Centre for Drug Discovery and Design. [2013, January]
Aurelie Chanson: A previous senior research assistant (2010-2012) Aurelie has secured a position as a Research Project Officer with CSIRO Plant Industry in Brisbane where she will work on molecular-plant pathogen interactions in cereals. [2012, November]
Joseph O'Neill: For our undergraduate IMB summer (2011/2012) scholar, the summer 'holiday' is over and Joseph is going back to uni classes to complete his BSc degree. [2012, January]
Christina Delay: A past honours student (2011), Christina is off to Canberra to start a PhD at the Australian National University in plant molecular biology. [2011, November]
Kate Martinac: A past honours student (2011), Kate is starting work at QUT's research commercialisation arm bluebox. [2011, November]
Lab News Archive
Below is all lab news since November 2011. Apologies if some of the links are dead.
Wednesday, 7 September 2022: Joel Haywood's manuscript on statins, their target HMG-CoA reductase and its potential as a herbicide target was accepted today for publication by Nature Communications. Stayed tuned for updates and the pre-print is on bioRxiv.
Tuesday, 2 August 2022: We're hiring a post-doc to work on fungicide biotransformation using our OrbiTrap Exploris 120. Open to internationals, $103K-$122K p.a. + 17% super. Close 30 Aug. Fixed term, 3 years, full-time (with the possibility of extension). See this Tweet.
Tuesday, 12 July 2022: We have 2 projects open for PhD candidates at Curtin University. The projects are entitled "Agrochemical behaviour in planta" and "New herbicidal modes of action". They close on 18 August 2022. Tweeted: Herbicides; Fungicides.
Wednesday, 20 April 2022: Out today is structural work led by Joel Haywood that examines the first crystal structures of plant HMGR, the target of cholesterol medication, and how it can be expoited to give statins plant-specificity. Check out the preprint via the DOI or this Tweet.
Thursday, 26 May 2022: We're hiring a post-doc to work on fungicide biotransformation using our OrbiTrap Exploris 120. Open to internationals, $91,431-$98,028 p.a. + 17% super. Fixed term, 3 years, full-time (with possible extension to 5 years). See the advert or this Tweet.
Friday, 25 February 2022: Our first foray into machine learning led by Douglas Pires and David Ascher came out today in Briefings in Bioinformatics entitled "cropCSM: designing safe and potent herbicides with graph-based signatures". See this Tweet to read more and access it.
Saturday, 9 April 2022: Early online at Plant Communications is our crystal structure paper on the target of asulam, jointly first-authored by Grishma Vadlamani & Kirill Sukhoverkov. See what we learned from the first plant crystal structure for HPPK/DHPS via its DOI or this Tweet!
Thursday, 17 February 2022: Our lab and that of Keith Stubbs have been jointly awarded an ARC Linkage Project with DemAgTech Pty Ltd. See the press release by Curtin "Research project to find herbicide options for tackling resistance" or this Tweet by me or the Tweet by Curtin.
Thursday, 6 January 2022: Our paper on linezolid as a herbicide "Inhibition of chloroplast translation as a new target for herbicides" led by PhD student Kirill Sukhoverkov got selected for the current cover of RSC Chemical Biology. See this Tweet to see it!
Saturday, 13 November 2021: Today on bioRxiv is joint-first author work by Grishma Vadlamani & Kirill Sukhoverkov "Crystal structure of Arabidopsis thaliana HPPK/DHPS, a bifunctional enzyme and target of the herbicide asulam". See more at its DOI, PDB 7MPY or this Tweet.
Friday, 17 September 2021: PhD student Colton Payne, based in Rosengren lab, has led two papers on unusual cyclic peptides with two disulfides; one in Chemical Science and a second today in RSC Chemical Biology on PDP-23 and PDP-24. Also see this April, 15 Tweet!
Tuesday, 14 September 2021: Work led by PhD student Michael Wallace from the Stubbs lab used a fluoroquinolone collection from Ulrich Jordis of TU Wien to publish his "Herbicidal activity of fluoroquinolone derivatives" in Plant Direct. Find it using its DOI or see this Tweet.
Wednesday, 14 July 2021: Two CCDM Scholarships are open for PhD candidates at Curtin University. The projects on Understanding agrochemical behaviour inside plants closes 12 December 2021 and is open to Australian Citizens and Australian Permanent Residents. Also see this Tweet.
Monday, 12 July 2021: We have 2 projects open for PhD candidates at Curtin University. The projects Agrochemical behaviour in planta and New herbicidal modes of action close 30 Aug. Tweeted: Herbicides; Fungicides.
Friday, 9 July 2021: MS/MS technician job vacancy. Up to $89K +17% super, 3 yr, FT. Maintain WA’s first OrbiTrap Exploris 120. Close 6 Aug. Must have the right to live and work at Curtin to apply. See advert on Seek, Curtin or Job active. See Tweet.
Tuesday, 11 May 2021: Plant Cell paper published "Structural and biochemical analyses of concanavalin A circular permutation by jack bean asparaginyl endopeptidase", see it by its DOI, Tweet and there's a wonderful Plant Cell First Author profile of Sam!
Tuesday, 20 July 2021: Out today in bioRxiv is Colton Payne's penultimate bit of PhD work "Structural characterization of the PawL-derived Peptide family, an ancient subfamily of orbitides", see it via its DOI and a summary at an accompanying Tweet.
Thursday, 18 March 2021: With mixed sadness and excitement I'm leaving UWA to join Curtin (also in Perth) as a Professor and the new Deputy Director of the Centre for Crop & Disease Management, see the Tweet and press release.
Monday, 8 March 2021: A perspective led by Dr Joel Haywood in Pest Manag. Sci. is out. What lessons for combatting herbicide resistance could be learnt from the global efforts to avert the superbug crisis. See via DOI and Tweet.
Wednesday, 24 February 2021: Out in RSC Advances, Kirill analysed antimalarials, refined what physchem 'rules' make a good herbicide. He found MMV1206386 which BASF say has a new mode of action, see it by its DOI and Tweet.
Friday, 30 October 2020: Today a lay-article came out in ASBMB Today magazine. Written by Laurel Oldach from USA's ASBMB, it explains how we redressed the literature with our evolidine project spanning 3 Australian states and 4 unis and published it in JBC. The work was in JBC was led by Mark Fisher.
Wednesday, 28 October 2020: Today Kirill's first, first-author manuscript from UWA came out in bioRXiv. "Refining physico-chemical rules for herbicides using an antimalarial library" was done in collaboration with BASF, Germany. See Tweet.
Tuesday, 20 October 2020: We have just made available some RNA-seq data generated from rare lycopod species, sourced as a collaboration with Jill Harrison of Bristol Uni. The species are Trithuria bibracteata and Phylloglossum drummondii. See the data at NCBI's SRA. This joins RNA-seq data for several Selaginella species as well as Isoetes drummondii published a couple years ago in James et al. (2017). Also Jill Harrison posted about these lycopod datasets in July 2016 as they were being gathered at her lab blog.
Thursday, 20 August 2020: Out today in JBC is work led by Mark Fisher "The genetic origin of evolidine, the first cyclopeptide discovered in plants, and related orbitides", see it via its DOI and a summary by PEB or an accompanying Tweet.
Wednesday, 17 June 2020: News from lab alumna, Dr Jinging Zhang has made the most of sudden border closures by landing a 3-year post-doc at Shenzhen hospital to research plant metabolites plus some human medicinal work too, see Tweet.
Saturday, 13 June 2020: Out today in bioRxiv is Mark Fisher's last PhD work "The genetic origin of evolidine, the first cyclopeptide discovered in plants, and related orbitides", see it via its DOI and a summary at an accompanying Tweet.
Friday, 29 May 2020: Today the first, first-author manuscript for UQ PhD student Colton Payne came out in bioRXiv. It's NMR-centric work on helical hairpin peptides. Colton is based in the Rosengren lab at UQ.
Monday, 11 May 2020: Today Dr Karen Breese, an organic synthetic chemist, joined UWA to work on herbicides funded by an ARC DP19. With a PhD from ANU and industry experience at Epichem, she's based in the Stubbs lab. Welcome!
Friday, 3 March 2020: Out today in J. Natural Products led by ex-PhD student Mark Fisher is the paper "Cyclic peptides in seed of Annona muricata are ribosomally synthesized", see it on PubMed or via its DOI.
Tuesday, 28 February 2020: Congratulations to new PhD student Victoria Cornford who landed a generous GRDC Research Scholarship "Assessing a sterol pathway component as a novel target for herbicides", see the Tweet.
Monday, 17 February 2020: The lab is chuffed to have Kalia Bernath-Levin back working for a year, bringing her protein expertise and directed evolution skills to some of the lab's Nexgen Plants projects.
Tuesday, 28 February 2020: Congratulations to our new PhD student Victoria Cornford lab who was told today she's landed one of the generous GRDC Research Scholarships for her project "Assessing a sterol pathway component as a novel target for herbicides", see Tweet.
Monday, 17 February 2020: The lab is chuffed to have Kalia Bernath-Levin back working for a year, bringing her protein expertise and directed evolution skills to some of the lab's Nexgen Plants projects, see Tweet.
Wednesday, 12 February 2020: We're hiring! We are looking for a post-doc synthetic organic chemist to make herbicides for 18 months at Level A8, see [link removed]. Closing date: Thursday, 5 March 2020.
Thursday, 19 December 2019: Led by UCSD, we co-authored on a paper out today in Cell Systems "De novo peptide sequencing reveals many cyclopeptides in the human gut and other environments". CycloNovo is a great program. Check it out via its DOI at Cell Systems, or if you hit the paywall, see bioRxiv.
Friday, 13 December 2019: Congratulations to the lab's Victoria Cornford, who not only got a great First Class Hons grade, but today was told she won a PhD scholarship on the same day as her UWA graduation day.
Monday, 2 December 2019: A colossus in the field of NMR (H-index >150, >115K cites), Ad Bax, came to Perth and talked at UWA on "Protein folding and Alzheimer’s Abeta peptide oligomerization viewed by rapid pressure jump NMR".
Friday, 25 October 2019: Josh has started Tweeting the limericks he uses each Friday, to attempt to remind lab members to do their weekly lab duties. Follow @j_mylne on Twitter or see the Limericks all at once at #LabLimerick.
Thursday, 3 October 2019: A bioRxiv manuscript we're involved in and led by UCSD scientists "De novo peptide sequencing reveals a vast cyclopeptidome in human gut and other environments" has now gone through peer review and accepted by Cell Systems. More info coming soon.
Tuesday, 1 October 2019: A manuscript "Sequencing orbitides by acid-mediated ring cleavage followed by tandem mass spectrometry" was posted online early at the Journal of Proteome Research website. To access it, click here.
Thursday, 8 August 2019: Our paper "An orbitide from Ratibida columnifera seed containing 16 amino acid residues" came out today in the Journal of Natural Products. To get it, go to this link for the DOI.
Friday, 26 July 2019: Today the ASBMB Special Interest Group "Perth Protein Group" was launched by its innaugural chairperson Josh with about 55 protein scientists from all over Perth. Follow @PerthProteins on Twitter.
Tuesday, 9 July 2019: Thanks Glenn Pullela, Matt Piggott and colleagues for including our work with them on "Total synthesis of the antimalarial ascidian natural product albopunctatone" out today in Organic Letters, access by its DOI.
Friday, 26 July 2019: Ringmaster of MS/MS Mark Fisher has done it again with a manuscript on the biggest known orbitide accepted today by Journal of Natural Products, more info coming soon.
Wednesday, 3 July 2019: Today Mark Fisher had his viva voce with Ray Norton of Monash Uni. and Louis Luk of Cardiff Uni. and passed with flying colours. It's Dr Fisher now!
Friday, 28 June 2019: Mark Fisher gave his PhD swansong (completion seminar) in front of the School today and nailed it! Next Wednesday he defends his thesis in a viva voce.
Wednesday, 26 June 2019: A manuscript on the antimalarial albopunctatone by Glenn Pullela of Matt Piggott's lab (on which Josh and Kirill co-author) was accepted today by Organic Letters. More info coming soon!
Friday, 14 June 2019: Congratulations Jingjing Zhang, who today submitted her PhD thesis entitled "The investigation of two buried peptide families as well as an exploration into new herbicides".
Wednesday, 12 June 2019: Congratulations to DECRA Fellow Joel Haywood and his wife Jojo on the birth of their son, Blake, who came into the world yesterday at a healthy 7 lb 2 oz or 3.24 kg in new money!
Saturday, 25 May 2019: See our latest pre-print on bioRxiv led by PhD student Mark Fisher and entitled "Novel cyclic peptides in seeds of Annona muricata are ribosomally synthesised".
Thursday, 23 May 2019: Out today is work by ex-PhD student Dr Ben Pouvreau (now at CSIRO in Canberra). "An interstitial peptide is readily processed from within seed proteins" is available with no paywall until 12 July 2019 or via its DOI.
Friday, 17 May 2019: Today, the lab welcomed Dr Grishma Vadlamani who hails from Monash University and will work on our recently funded DP19 on novel herbicide targets.
Friday, 10 May 2019: Mark Fisher will have his PhD viva voce on Wednesday 3 July and two examiners will fly in for it plus give talks in our School's seminar series, namely rising star Dr Louis Luk of Cardiff University and the legendary Prof. Ray Norton of Monash University.
Thursday, 2 May 2019: A manuscript led by Ben Pouvreau entitled "An interstitial peptide is readily processed from within seed proteins" was accepted by Plant Science. Synthetic approaches showed how easily unrelated seed proteins can make the usually albumin-buried SFTI peptide.
Tuesday, 30 April 2019: Congratulations to PhD student Mark Fisher who today submitted his PhD thesis, which is in the format of thesis by publication including 5 published works, one in bioRxiv, two under review and one in preparation - a productive PhD!
Thursday, 11 April 2019: A second example of protein hijack is out "An ancient peptide family buried within vicilin precursors" came out today in ACS Chemical Biology. You can access it via its DOI or via PubMed .
Thursday, 21 February 2019: Our paper "The macrocyclizing protease butelase 1 remains auto-catalytic and reveals the structural basis for ligase activity" came out today in The Plant Journal. To get it, go to this link for the DOI.
Tuesday, 19 February 2019: Anyone wanting to get their mitts on our construct for making supercycliser butelase 1 (C. ternatea AEP1) in E. coli, it's available at Addgene.
Wednesday, 16 January 2019: Work we helped with is just out at BioRxiv. The CycloNovo algorithm developed at UCSD by Pavel Pevzner and colleagues does de novo cyclopeptide discovery and uniquely uses de Bruijn graphs, common in NGS assembly.
Tuesday, 27 November 2018: The Australian Research Council has awarded us two Discovery Project grants. One grant aims to discover plant processes and plant proteins that can be the target for new herbicides. The other aims to examine how evolution in plants shortcuts the creation of new proteins by burying one within another.
Thursday, 7 February 2019: Our manuscript "The macrocyclizing protease butelase 1 remains auto-catalytic and reveals the structural basis for ligase activity" was just accepted by The Plant Journal. It is available now at BioRxiv and more news coming soon.
Friday, 11 January 2019: A grant-funded stipend to ($30,000 p.a. tax-free, 3 years) to work on herbicide target discovery is available, details at goo.gl/5zC2r4, applications close 18 February 2019.
Thursday, 10 January 2019: We have a 3 year post-doc position ($92,497 p.a. + 17% super.) to work on herbicide target proteins, see the UWA job #502578, applications close 15 February 2019.
Monday, 17 December 2018: Congratulations to Dr Joel Haywood, who won a 2019 Thomas Davies Research Grant for Marine, Soil and Plant Biology worth $15,000 from the Australian Academy of Science.
Wednesday, 14 November 2018: Congratulations to Bingsen Zhang, who was offered a PhD scholarship by UWA. Bingsen is currently completing a degree in the School of Chemistry and Chemical Engineering at Nanjing University, so if all goes to plan, he will start a PhD here in 2019.
Monday, 29 October 2018: The lab welcomes Mario Bani who started his PhD today. Mario did his MSc at the Institute for Molecular Bioscience at UQ with Prof. Glenn King working with recombinant protein venoms. He will work on novel herbicide targets genetically and with biochemical approaches.
Monday, 1 October 2018: We are helping organise a conference at UWA 15-17 January 2020 entitled "Herbicide Discovery & Development" (HDD2020). See the website and follow it on Twitter and use the hashtag #HDD2020.
Thursday, 16 August 2018: The structural co-ordinates for the macrocyclizing protease butelase 1 were released by the Protein Data Bank today with the accession number 6DHI.
Friday, 10 August 2018: Check out this interview (YouTube or MP3 download) by Josh with the Laymans Podcast; two young guys interested in science and communication, operating from their parents' back garden shed.
Tuesday, 31 July 2018: We have nervously entered the preprint world with our manuscript "The macrocyclizing protease butelase 1 remains auto-catalytic and reveals the structural basis for ligase activity" published today on bioRxiv.
Monday, 9 July 2018: The lab welcomes Zhang Bingsen from Nanjing University (China) who will stay for a two-month research placement as part of the UWA International Research Internship Program (IRIP).
Friday, 8 June 2018: Our paper in Plant Journal "Targeting plant DIHYDROFOLATE REDUCTASE with antifolates and mechanisms for genetic resistance" came online today in early view format and can be found by its DOI or in PubMed.
Thursday, 24 May 2018: Congratulations again to Max Corral whose PhD thesis “Antimalarial herbicides and herbicidal antimalarials: Exploiting the plant-Plasmodium connection” was accepted by the UWA Board of the Graduate Research School as satisfying the requirements for the degree of Doctor of Philosophy today!
Tuesday, 22 May 2018: Congratulations Max Corral and colleagues, whose work on DHFR as a herbicide target was just accepted today for publication by The Plant Journal. More information coming soon.
Friday, 6 April 2018: The academic promotions committee at UWA notified Josh Mylne today that he was promoted from Senior Lecturer to Associate Professor, effective 13 March 2018!
Friday, 16 March 2018: Congratulations Amy James whose thesis “Evolutionary insights into two plant protein families: Bowman-Birk inhibitors and asparaginyl endopeptidases” was accepted today by the UWA Board of Graduate Research School for the degree of Doctor of Philosophy!
Thursday, 22 February 2018: Our School of Molecular Sciences is on the hunt to fill a new synthetic biology position at Level C (senior lecturer) or Level D (Associate Professor). Applications close 2 May 2018.
Friday, 16 February 2018: Check out the article by RSC Publishing Editor Olivia Wakeman "Antibiotic analogue puts researchers on path to ending herbicide drought" in Chemistry World about our paper in ChemComm.
Tuesday, 13 February 2018: Our Schools's new macromolecular X-ray suite (a Rigaku Oxford Diffraction XtaLAB Synergy-S diffractometer) has arrived and is being installed this week by Phil O'Donnell from AXT. Huzzah!
Monday, 12 February 2018: We just found out that Bastian Franke's review in Natural Product Reports got the cover with some eye-catching art ... stay tuned!
Wednesday, 7 February 2018: After a small delay, the PDB file "Asparaginyl endopeptidase 1 bound to AAN peptide, a tetrahedral intermediate" associated with the paper in eLife is released at PDB under accession 6AZT.
Thursday, 1 February 2018: Just out today in eLife, our paper by Joel Haywood et al. entitled "Structural basis of ribosomal peptide macrocyclization in plants". Read it now via its DOI or see the press release.
Friday, 26 January 2018: What a great week for the lab, with 5 papers accepted in 7 days. Post-doc and soon to be DECRA Fellow Joel Haywood had his first-author manuscript on the crystal structure of active sunflower AEP accepted at eLife. It is available by its DOI.
Thursday, 25 January 2018: Good things come in fours? Joint PhD student Michael Wallace just had his manuscript on ciprofloxacin analogues and their herbicidal activity accepted at RSC journal Chemical Communications. See it using its DOI.
Saturday, 20 January 2018: Good things come in threes ... PhD student Mark Fisher had his lead-author manuscript revealing 50 peptide rings (called PLPs) accepted today at the new open-access journal Plant Direct. More information coming soon.
Friday, 19 January 2018: A doubly good day ... we just had a manuscript on albumin-buried peptides led by ex-PhD student Bastian Franke (based in the lab of Dr Johan Rosengren) accepted today at the high-impact review journal Natural Product Reports. It is accessible by its DOI.
Friday, 19 January 2018: On his last day as a PhD student, Max Corral's manuscript on a herbicidal antimalarial was accepted by Pest Management Science. This paper expands our lab's work on herbicides done in collaboration with Assoc. Prof. Keith Stubbs and with BASF in Germany. It is available via DOI or PubMed.
Thursday, 21 December 2017: Josh has won a prestigious Fulbright Professional Scholarship to visit the University of Minnesota St Paul where he'll be hosted by Asst Prof. Mikael Elias for 3 months including December 2018 until February 2019. UWA issued a press release detailing its 2018 winners.
Tuesday, 19 December 2017: Congratulations Maxime Corral who today submitted his PhD thesis entitled "Antimalarial herbicides and herbicidal antimalarials: exploiting the plant-Plasmodium connection" for examination.
Monday, 18 December 2017: What timing! Just days before submitting his PhD thesis, lab member Max Corral has secured a 3-yr CSIRO Postdoctoral Fellowship in Floreat, Perth to work on novel technologies for control of crop diseases with Dr Jonathan Anderson.
Tuesday, 21 November 2017: Today at 10:30am, Josh is giving a seminar for the Plant and Environmental Sciences Department at the Weizmann Institute of Science entitled "Antimalarial herbicides and herbicidal antimalarials: exploiting the plant-Plasmodium connection"
Tuesday, 14 November 2017: At the Weizmann Institute of Science's Biomolecular Sciences Department today, Josh delivered a special guest lecture "The Fellowships of the Rings: evolution and biosynthesis of circular peptides"
Friday, 10 November 2017: Congratulations to post-doc Dr Joel Haywood who was awarded a 3-year ARC DECRA Fellowship "Structure-based investigations into new modes of action for herbicides".
Wednesday, 1 November 2017: A lay article by Josh on the history leading to recent work on antimalarial herbicides out in Sci. Rep. and Angewandte Chemie featured in the September issue of Australasian Science magazine. The article "Malaria Drugs Offer New Herbicide Targets" can now be downloaded. Click here for the PDF.
Monday, 30 October 2017: Congratulations to Amy James who today submitted her PhD thesis entitled "Evolutionary insights into two plant protein families: Bowman-Birk inhibitors and asparaginyl endopeptidases" for examination. The floppy hat beckons!
Wednesday, 20 September 2017: A grant application led by CSIRO's Jonathan Anderson and partnered with our lab was successful. The goal is to develop peptides that transport RNA for plant protection and a 3-year fellow will be employed to work on this at CSIRO in Floreat.
Monday, 3 July 2017: Our recent work on antimalarial herbicides was picked up by ABC who covered it on FaceBook, in an online article and in a podcast interview with Josh that starts about 9 minutes in (span is 09:20-15:21).
Tuesday, 27 June 2017: Congratulations to PhD student Max Corral whose manuscript "Exploiting the evolutionary relationship between malarial parasites and plants to develop new herbicides" came online at Angewandte Chemie today. This work was a close collaboration with Assoc. Prof. Keith Stubbs, an organic chemist in our School of Molecular Sciences. It can be viewed via PubMed, its DOI and this is the press release.
Wednesday, 24 May 2017: Out today in JBC is a lovely triple resonance NMR structure of one of our favourite proteins, PawS1, done in collaboration with Dr Johan Rosengren from UQ and led by (now ex-) joint PhD student Bastian Franke who's begun a post-doc in Switzerland. The paper "Two Proteins for the Price of One: Structural Studies of the Dual Destiny Preproalbumin with Sunflower Trypsin Inhibitor-1" can be accessed via its DOI or PubMed.
Monday, 22 May 2017: WA mining mogul Andrew Forrest and his wife Nicola have made a second philanthropic $65 million donation to the Forrest Research Foundation building on their generous donation in 2013. Their donations benefit all five Western Australian universities and represent the largest contribution by private individuals to Australian higher education.
Tuesday, 4 April 2017: Our work on antimalarial herbicides was highlighted in the latest edition (Issue 22) of Leading Agriculture, a "digital magazine showcasing the best in Australian farming".
Monday, 3 April 2017: Our paper highlighting the close relationship between malaria and plants "Herbicidal properties of antimalarial drugs" is now out in Scientific Reports and a press release may be viewed here.
Thursday, 30 March 2017: Our artwork for the recently published paper on BBIs was selected by The Plant Cell for the March cover! The art, done by Scot Nicholls of Domokun Design will be his second work to grace the cover of Plant Cell. Scot's art was also featured on the Plant Cell March 2014 cover.
Friday, 24 March 2017: Over 110 new transcriptomes were used to the birth and evolution of SFTI-1 in our new Mol. Biol. Evol. paper that was indexed today in PubMed and the article "Stepwise evolution of a buried inhibitor peptide over 45 million years" by Achala Jayasena et al. can be viewed using its DOI. A press release also just came out.
Wednesday, 22 March 2017: A short review on AEPs authored with PhD student Amy James and Dr Joel Haywood just went live in the Wiley Online Library, click DOI to access it at New Phytologist or go via PubMed.
Wednesday, 15 March 2017: Our paper by Amy James and colleagues just came online at The Plant Cell: "Evidence for Ancient Origins of Bowman-Birk Inhibitors from Selaginella moellendorffii". Click for Press Release or go straight to the paper by DOI.
Tuesday, 7 March 2017: They say that good things come in 3s! We're delighted that a manuscript that solves a long-standing mystery regarding the provenance of Bowman-Birk Inihibitors was accepted recently by The Plant Cell. The work was spear-headed by PhD student Amy James and involved collaboration locally with Charlie Bond and at La Trobe with Jim Whelan and colleagues. Stay tuned for updates!
Saturday, 4 March 2017: Congratulations to PhD student Max Corral whose manuscript on an interesting connection between plants and human parasites was accepted by Scientific Reports today. His paper will signal the beginning of a new line of research by our lab into herbicides. This work is done in collaboration with Assoc. Prof. Keith Stubbs, an organic chemist in our School of Molecular Sciences. More information coming soon.
Friday, 3 March 2017: This afternoon we're breaking out the beers to celebrate a manuscript recently accepted by the journal Molecular Biology and Evolution. The work by PhD student Achala Jayasena and others from the lab draws on a staggering 107 new transcriptomic datasets acquired in collaboration with Jim Whelan and colleagues at La Trobe and phylogenies by Jose Panero and Ed Schilling from the USA. Its puts together the genetic steps that over 45 million years evolved the inhibitor peptide SFTI-1. More information coming soon.
Monday, 27 February 2017: Welcome to Luca Stehl who started his lab placement today as part of an exchange between UWA and his alma mater, Freiburg University in Germany, where he is pursuing a Masters in Plant Biology. Luca will learn recombinant protein production working with potential new herbicide targets.
Monday, 20 February 2017: The lab welcomes Samuel Nonis who started his PhD today. Sam did his Honours at UWA, had an internship at A*Star Institite in Singapore before winning a prestigious IPRS scholarship. He will work on proteases and protein evolution.
Wednesday, 25 January 2017: Congratulations Amy James and Joel Haywood whose Tansley Insight on ring-making proteases from plants was accepted today by New Phytologist. More details to come once the article become available.
Tuesday, 17 January 2017: Dr Pouvreau it is! Today Ben's thesis entitled “The genesis of new proteins and new biosyntheses” was accepted by the Board of the Graduate Research School as satisfying the requirements for a Doctor of Philosophy.
Friday, 6 January 2017: We're in! Today we moved lock, stock and barrel one floor down to lab 2.55 in the Bayliss Building alongside the labs of Mark Howard, Heng Chooi and Mark Waters.
Monday, 19 December 2016: Congratulations to Michael Wallace who won a scholarship today to pursue a PhD in 2017 with close collaborator Assoc. Professor Keith Stubbs. Michael will continue the work he did during honours on herbicides in collaboration with our lab.
Friday, 16 December 2016: The lab is moving! Today we were given the keys to lab 2.55 also in the Bayliss Building. With a whopping 170 square metres of floor space as well as equipment rooms and walk-in coldroom it's quite the upgrade from our lab on the third floor of Bayliss. We will share our lab with new NMR appointee Dr Mark Howard and our lab is adjacent to those of ARC Future Fellows Dr Heng Chooi and Dr Mark Waters. A great micro environment for chemical biology research!
Tuesday, 22 November 2016: If you're heading to the 2017 42nd Lorne Conference on Protein Structure and Function, Josh will be talking in the "Movement and evolution" session on Monday 6 February on "Buried treasure: the de novo evolutionary birth of proteins from within a host".
Monday, 10 October 2016: Our paper by ex-PhD student Alysha Elliott and joint PhD student Bastian Franke entitled "Natural structural diversity within a conserved cyclic peptide scaffold" is now available via PubMed or following its DOI.
Saturday, 10 September 2016: After a long wait, Achala Jayasena finally gets to don the floppy hat for her PhD thesis entitled "De novo birth and stepwise evolution of a seed peptide". Achala started her PhD in 2012 and has authored papers in Biopolymers, TAG, Chem. Biol., Plant Methods and Plant Cell with more on the way!
Friday, 9 September 2016: Congratulations to ex-PhD student Alysha Elliott and joint PhD student Bastian Franke, whose manuscript on NMR structures of some buried peptides was accepted today for publication in Amino Acids, more details soon.
Friday, 29 July 2016: Today Bayer CropScience announced we had won a grant from their scheme Grants4Targets. The proposal "Ripping plant plastid DNA replication in two" will develop gyrase inhibitors as herbicides in collaboration with the Stubbs lab and Prof. Tony Maxwell of the John Innes Centre in the UK.
Monday, 18 July 2016: We're sharing RNA-seq datasets for three rare plants at key phylogenetic nodes; the lycophytes Phylloglossum drummondii and Isoetes drummondii and the basal angiosperm representative Trithuria bibracteata. For details see this blog by collaborator Jill Harrison from Bristol University.
Thursday, 14 July 2016: An easy-to-read article about cleavage coupled intramolecular transpeptidation has been reprinted in this month's issue of Chemistry in Australia magazine, click here to download the PDF. Also, check out Chemistry in Australia magazine on-line - it's a good read!
Tuesday, 12 July 2016: Congratulations to Benjamin Pouvreau who just handed in his PhD thesis entitled "The genesis of new proteins and new biosyntheses" for examination. Party time this Friday!
Monday, 11 July 2016: Congratulations to Achala Jayasena whose PhD thesis entitled “De novo birth and stepwise evolution of a seed peptide” has been accepted by the Board of the Graduate Research School as satisfying the requirements for the degree of Doctor of Philosophy in this University. Floppy hat ceremony is next!
Friday, 1 July 2016: A publication on seed peptides from Zinnia came out on-line at Biopolymers today; congratulations to lead author Bastian Franke and co-authors from our lab, Achala and Mark. It can be accessed via its DOI or at PubMed.
Friday, 24 June 2016: Great news for PhD student Benjamin Pouvreau, who just landed a 3-yr CSIRO Postdoctoral Fellowship to work on canola oil biosynthesis in Canberra. He's now more motivated than ever to submit his thesis!
Monday, 20 June 2016: The lab welcomes Joel Haywood who today starts a post-doc that will bring protein structure to some of the biosyntheses and bioactivities our lab is interested in. Joel just finished his PhD at the Chinese Academy of Science working on leukocyte receptors.
Thursday, 16 June 2016: Congratulations to Ben Pouvreau who gave his PhD completion seminar today. To celebrate the lab's RA Julie baked Ben some special Arabidopsis cupcakes, see them in the banner here.
Saturday, 7 May 2016: Congratulations to PhD student Bastian Franke, whose manuscript on the structures of the major sunflower albumins was accepted today by the Journal of Proteomics for an upcoming special on Foodomics, more details soon.
Saturday, 2 April 2016: We're advertising a 2-year post-doc position to work on the biosynthesis of bioactive peptides by proteases. Those with recombinant protein and mass spec experience will be favoured. Closes Wednesday 27 April [LINK REMOVED].
Wednesday, 16 March 2016: A lay article by Josh on a fascinating cleavage-coupled intramolecular transpeptidation reaction called "Protein scissors that also learned to glue" is now out in Australasian Science magazine, click here to download the PDF.
Monday, 29 February 2016: Today the lab welcomes Brodie Ketteridge-Hall who will start his honours working on ligating proteases as well as joint Honours student Michael Wallace who will be based in the Stubbs lab working to turn ciprofloxacin into a better herbicide.
Friday, 12 February 2016: A press release for a joint JBC paper on plant DNA gyrase with a team UK came out today: "Common antibiotic inspires hunt for new herbicide". Click for Press Release or go straight to the paper by DOI.
Monday, 18 January 2016: Our paper "A tripartite approach identifies the major sunflower seed albumins" by outgoing PhD student Achala Jayasena and collegues from UQ has just come out on-line at Theor. Appl. Genet. and can be accessed via PubMed or its DOI.
Thursday, 14 January 2016: Josh will be visiting the UK in April; he will be in Oxford 9-12 April and speak at Plant Proteases 2016 then in Norwich 13-19 April and will speak at the John Innes Centre on 18 or 19 April.
Monday, 11 January 2016: Our lab advertised a PhD stipend to pursue the enzymatic basis for the production of a bioactive peptide. Applications close 20 January 2016. Details may be found here.
Friday, 18 December 2015: Congratulations to the lab's Mark Fisher who today was awarded the Bruce and Betty Green Postgraduate Research Scholarship to be held in conjunction with his Australian Postgraduate Award while doing his PhD.
Wednesday, 9 December 2015: A manuscript for work done by our lab's Julie Leroux in collaboration with Prof. Tony Maxwell at the John Innes Centre (UK) was published on-line today by The Journal of Biological Chemistry.
Monday, 7 December 2015: Today the lab welcomed undergraduate summer studuents Morgan Biggs and Conor MacKay who will work with PhD candidate Amy James producing sunflower proteases to skill up on recombinant protein production and purification. Welcome boys!
Thursday, 5 November 2015: Congratulations to PhD students Achala Jayasena and Bastian Franke, whose paper identifying the major sunflower seed albumins was accepted today by Theoretical and Applied Genetics.
Friday, 30 October 2015: The Australian Research Council has awarded us a Discovery Project grant to study how a protein bond breaker evolved to also be a protein bond maker. This will allow new insight into forces that shaped enzyme evolution and enable the design of enzymes with superior cutting or ligating ability for industrial applications.
Monday, 27 October 2015: Today the lab welcomed the arrival of Jingjing Zhang who begins her PhD on bioactive peptide processing in sunflower seeds. Jingjing obtained her MSc. in biophysics from the University of Chinese Academy of Sciences and won a prestigious IPRS scholarship. Details of scholarships offered for PhD study at UWA are available here.
Friday, 23 October 2015: Check out this interview of Josh for 42evolution.org ... a box of steak knives to anyone who can work out what the gaff just before 2.00 was. Check out other interviews here, including luminaries such as Sir David Attenborough, George McGhee, Simon Conway Morris and many more!
Wednesday, 16 September 2015: Alongside close collaborator Keith Stubbs, Josh was a recent recipent of a Vice-Chancellor’s Mid-Career Research Award announced at a recent ceremony during UWA Research Week.
Friday, 11 September 2015: In one week Edward E. Schilling, a world expert in sunflower systemists from The University of Tennessee (Knoxville, USA) will arrive for a working visit from Fri, 18 September – Mon, 5 October.
Monday, 17 August 2015: Past PhD student Dr Alysha Elliott has been nominated for the Prestigious Centenary Institute Lawrence Creative Prize for her work attempting to find the next big antibiotic against superbugs through the Community for Open Antimicrobial Drug Discovery initiative. Vote for her in the people's choice award here.
Monday, 17 August 2015: After blitzing his undergraduate and honours years, Mark Fisher went on to win an APA and started his PhD today. He will be attempting to elucidate the frequency with which proteins are born inside one another.
Wednesday, 5 August 2015: Today the lab welcomes Andrew McLagan who is beginning a PhD on herbicide discovery. Andrew has honours in synthetic organic chemistry and in addition to doing all his own compound testing will be busy in the hood in a joint project with the lab of Assoc. Prof. Keith Stubbs also from UWA’s School of Chemistry & Biochemistry.
Friday, 24 July 2015: Congratulations to Jingjing Zhang who won a prestigious IPRS scholarship from UWA to start her PhD in August. Jingjing has a M.Sc. from The University of Chinese Academy of Sciences and has been working for the Beijing Genomics Institute. Her project will focus on peptide biosynthesis and biodegradation. Details of scholarships offered for PhD study at UWA are available here.
Friday, 3 July 2015: Today we said farewell to Ellen Adams from Cleveland Ohio (USA) who joined the lab for a 4 week stay. Just two days ago she got her sequence back and found she'd cloned her novel PawL1 gene successfully!
Friday, 19 June 2015: We are helping organise a hands-on, 2-day beginners-level workshop for analysis of transcriptomes which will be held at La Trobe University as a satellite to the 2015 Melbourne ComBio conference. Click [LINK REMOVED BY JSM] to visit the workshop website. There's only 25 places and to register is $50 so apply ASAP!
Tuesday, 9 June 2015: Today Ellen Adams from Cleveland Ohio (USA) joined the lab for a 4 weeks stay, during which time she will study the step-wise evolution of peptides by tracing the history of the PawS1 gene.
Thursday, 7 May 2015: Our paper "Peptide Macrocyclization by a Bifunctional Endoprotease" by Bernath-Levin et al. came out today in Chemistry & Biology and will feature on the cover of their May issue. See the accompanying press release.
Friday, 1 May 2015: We just heard the artwork submitted to accompany our manuscript on cleavage-coupled transpeptidation by Bernath-Levin et al. was chosen for the cover of the May issue of Cell Press's Chemistry & Biology, more details coming soon.
Friday, 3 April 2015: Our manuscript by Bernath-Levin et al. illustrating how an endoprotease is able to cleave as well as ligate in a sunflower cyclic peptide biosynthesis was accepted today by Cell Press's Chemistry & Biology, more details soon.
Thursday, 2 April 2015: Our Organic & Biomolecular Chemistry paper done collaboratively at UWA with Keith Stubbs and Michael Gandy to develop an interactive herbicide database was released on-line today and may be accessed via its DOI. To download the database itself (i.e. the .zip file), click here.
Monday, 2 March 2015: Yen Yeen Chor, a post-grad student in the first cohort of the new UWA Masters of Biotechnology joined the lab today and will work part-time on an international project with U. Texas that will refine phylogenies of some daisies.
Tuesday, 17 February 2015: Ellen Adams from Cleveland Ohio (USA) will be coming to the lab in June for 4 weeks as a summer (err, well actually winter) student, during which time she will study the step-wise evolution of peptides by tracing the history of the PawS1 gene.
Wednesday, 4 February 2015: We have word that two world expert sunflower systemists Jose L. Panero (U. Texas, USA) and Edward E. Schilling (U. Tennessee, Knoxville) from USA are planning a working visit to our lab later this year in spring. Will post again when dates are confirmed.
Friday, 9 January 2015: Congratulations to Shohidul Alam, who has been awarded his PhD from UQ entitled "Engineering Insect-Resistant Plants by Transgenic Expression of an Insecticidal Spider-Venom Peptide" and is now set-up in his office back in Bangladesh.
Monday, 1 December 2014: Today we welcomed a summer volunteer student Clarise Hewetson who will be learning the molecular biology ropes by engineering plants to answer evolutionary questions about a bifunctional protease.
Thursday, 13 November 2014: The raw data for the Heliopsis helianthoides and Zinnia haageana de novo seed transcriptomes in Jayasena et al. (2014) were registered with the NCBI BioProject database (PRJNA255932 and PRJNA255931) and deposited in the short reads archive (SRX659707, SRX659706), links to H. helianthoides and Z. haageana.
Wednesday, 29 October 2014: Our paper "Next generation sequencing and de novo transcriptomics to study gene evolution" by Jayasena et al. is now available online at Plant Methods.
Monday, 27 October 2014: A manuscript we helped with regarding the evolution of Momordica seed peptides was accepted today by Molecular Biology & Evolution, more details to follow. Congratulations Tunjung!
Saturday, 25 October 2014: A retrospective of the eclectic "Are there limits to evolution?" conference in Cambridge UK was blogged about recently at the Map of Life, a site dedicated to convergent evolution.
Thursday, 9 October 2014: The details of our deep sequencing project using specimens for three important taxa have been posted by Jill Harrison on the Annals of Botany AoB blog.
Wednesday, 8 October 2014: Our manuscript by Jayasena et al. that details how we assemble and test de novo transcriptomes for what is now over 100 non model organisms was accepted today by Plant Methods, more details coming soon.
Tuesday, 30 September 2014: Today the delivery of some live plants signalled the beginning of a collaborative project with Jill Harrison of Cambridge University and Kingsley Dixon of Kings Park and Botanic Garden to generate de novo transcriptomes for some deep time taxa at key phylogenetic nodes, namely the clubmoss Phylloglossum drummondii, the quillwort Isoetes drummondii and the basal angiosperm Trithuria bibracteata.
Thursday, 4 September 2014: A science communication article "A Protein Is Born" written by Josh is now out in the September issue of Australasian Science magazine. It provides a simpler explanation of the key findings from work published in March 2014 in Plant Cell. A PDF of it is here.
Monday, 11 August 2014: Today the lab welcomed Daisy, a Year 10 work experience student from Perth's Thornlie Christian College. She's here for a week to get a practical and theoretical grounding in genetics.
Monday, 16 June 2014: Congratulations to Shohidul Alam who submitted his PhD thesis to the UQ offices today regarding methods to engineer insect resistant plants. With any luck, in a few months Shohidul will get to wear that floppy hat!
Monday, 5 May 2014: Today Josh begins a month-long stint as a Feinberg Foundation Visiting Fellow in the Department of Biological Chemistry of the Weizmann Institute in Israel where he is being hosted by Dan Tawfik, a expert in enzyme mechanism and evolution.
Monday, 28 April 2014: PhD student Maxime Corral has been selected to represent UWA at James Cook University in Cairns later this year for the annual meeting of the Tropical Research Network, a collaborative network established with seven other Australian universities.
Friday, 25 April 2014: A science communication article "Genes that cuddle in the cold" written by Josh will feature on the cover of the May issue of Australasian Science magazine. It provides an informal prehistory and overview of work published in September 2013 in Genes & Dev. A PDF of it can be downloaded from here.
Wednesday, 9 April 2014: The raw data for the Arnica montana and Helianthus annuus de novo seed transcriptomes in Elliott et al. (2014) were registered with the NCBI BioProject database (PRJNA233448 and PRJNA233447) and deposited in the short reads archive (SRX455875, SRX450622), links to A. montana and H. annuus. The full 18 MB supplementary dataset for Elliott et al. (includes FASTA de novo transcriptomes, trees, NEXUS files and tables) may be downloaded from this link to Dryad
Friday, 28 March 2014: Our Plant Cell paper on the evolutionary history within the daisy familiy (Asteraceae) of a buried seed peptide went on-line today and may be accessed via its DOI or this toll-free link to it at Plant Cell. The associated press release is here.
Monday, 10 February 2014: Our manuscript that describes a new family of seed peptides and proposes its de novo evolution was accepted today by The Plant Cell, more details soon.
Wednesday, 22 January 2014: Welcome to Amy Franklin who started her PhD today. Amy just completed her Masters at the University of Victoria in Canada on the tannin biosynthesis pathway in poplar. She will work on an interesting potential case of structural and functional convergent evolution by a protein motif.
Monday, 13 January 2014: Welcome to Maxime Corral who arrived in the lab today, coming straight from Perth airport. A native of France, Max earned his Masters in plant biotechnology from the University of Otago where he worked on the response of clover to white clover mosaic virus. He embarks on the long haul to a PhD so he can don the floppy hat.
Thursday, 9 January 2014: Josh has been invited to speak at the "Are there limits to evolution?" conference at St John's College (Cambridge, UK) held later this year on 25-26 September.
Tuesday, 17 December 2013: The Australian Research Council announced today that the Centre of Excellence in Plant Energy Biology (to which our lab is affiliated) will receive $26 million in funding from 2014-2020. Congratulations CPEB2!
Tuesday, 10 December 2013: Today, recent UWA graduate Jared Murray was awarded a 2014 Grains Industry Undergraduate Honours Scholarship from the GRDC for his honours project entitled "The biochemical mode of action for members of a new stream of potential herbicides".
Monday, 28 October 2013: Congratulations also to Amy Franklin who was offered UIS and SIRF scholarships from UWA to start a PhD in early 2014. Amy just handed in her thesis for a Masters in Biology at the University of Victoria in Canada. Details of scholarships offered for PhD study at UWA are available here.
Friday, 25 October 2013: Congratulations to Maxime Corral who was today awarded UPAIS and SIRF scholarships from UWA to join us for a PhD. Max just completed his Masters in plant biotechnology at the University of Otago and will fly over from Pannes in France to start in early 2014. Details of scholarships offered for PhD study at UWA are available here.
Tuesday, 15 October 2013: Mining billionaire Andrew Forrest has just committed $65 million to universities in Western Australia. This welcome donation is one of the biggest single philanthropic donations in all of Australia's history. See this link at ABC for details.
Friday, 11 October 2013: We just received seed funding from UWA's Office of Industry & Innovation to explore a new research area on herbicides in collaboration with a group of chemists led by Assoc. Prof. Keith Stubbs.
Tuesday, 8 October 2013: Next year, Josh will visit the Department of Biological Chemistry at the Weizmann Institute for 1 month as a Feinberg Foundation Visiting Faculty Program Fellow where he will be hosted by Prof. Dan Tawfik, an expert in protein structure, biochemistry and evolution.
Friday, 6 September 2013: Ten years in the making, an approach that allowed monitoring the movement of an FLC gene within the nucleus of live Arabidopsis was published online today at Genes & Dev., see also the press releases from the JIC and UWA.
Tuesday, 13 August 2013: Ten years after the first genetic construct was made, our manuscript describing the physical movement of a gene targeted by vernalization was accepted for publication today by Genes & Development, more details soon.
Tuesday, 6 August 2013: Alysha Elliott got her thesis in the mail today and will don the floppy hat at the next PhD graduation cermony. Congratulations! Also Achala Jayasena and Ben Pouvreau's PhD research proposals were both accepted without change today by UWA ensuring their next significant milestones are theses.
Friday, 1 July 2013: German Masters graduate Bastian Franke from The Georg Simon Ohm University of Applied Sciences in Nuremberg will start his PhD today at UQ on a joint-project funded by a grant held by our lab and that of Dr Johan Rosengren. Bastian has a strong background in proteomics from an Internship at Bayer Health Care, USA. We wish him the best of luck.
Monday, 24 June 2013: The International Conference on Arabidopsis Research (ICAR) begins today in Sydney. Josh is talking at 10:45 in Friday's SYM-17. Come check out the giant inflatable plant cell too.
Tuesday, 18 June 2013: Congratulations to Alysha Elliott who today submitted her revised PhD thesis entitled "A new class of dasiy seed peptides" to the UQ offices. With luck she will soon be the proud wearer of a floppy hat!
Monday, 15 April 2013: Today the lab welcomes the arrival of Dr Kalia Bernath-Levin, hailing from Israel’s Weizmann Institute and the Technion. She begins a post-doctoral stint that will look at the evolution and biochemistry of proteases involved in peptide biosynthesis.
Monday, 18 February 2013: Our new RA Julie Leroux begins work today! Julie has a Masters from Paris VII University and has come to us from a four-year stint in the Laboratory of Reproduction and Plant Development in Lyon, Eastern France.
Monday, 5 February 2013: The lab welcomes travelling post-doc Claudia Rossig who obtained her PhD from the Universities of Grenoble (France) and Bayreuth (Germany). She will stay with us for a few weeks to help get the lab up, running and humming!
Thursday, 31 January 2013: Congratulations to Alysha Elliott, who just submitted her PhD thesis to the UQ offices! After just three days rest, she will start as a project manager with NHMRC Australia Fellow Professor Matt Cooper optimising an antibacterial screening pipeline for the Centre for Drug Discovery and Design.
Wednesday, 30 January 2013: Dr Kalia Bernath-Levin has agreed to join the group in March to bring expertise in protein design and directed evolution. Kalia did her PhD and first post-doc with Dan Tawfik at Israel’s Weizmann Institute before a post-doc with Ayelet Fishman at the Technion.
Wednesday, 19 December 2012: Our collaborative study of the structure of the Arabidopsis VRN1 protein and its DNA binding was published online today at JBC.
Friday, 23 November 2012: We're looking for a post-doc. See Positions Available for details. Closing date is Friday, 14 December 2012.
Monday, 6 November 2012: The Australian Research Council has awarded us a grant to study a diverse class of drug-like mini-proteins that are thought to have emerged genetically over 12 million years ago. This project will explore why plants have kept making these mini-proteins for so long and whether it is the same reason the founding member of this mini-protein class is such a good drug.
Wednesday, 17 October 2012: French engineer in plant biology Benjamin Pouvreau has been awarded prestigious IPRS and APA scholarships and will join the lab to begin his PhD in January 2013. Details of scholarships offered for PhD study at UWA are available here.
Wednesday, 10 October 2012: Our work was highlighted in the Australian Research Council Annual Report for 2011-2012, tabled today in Parliament in time for Senate Estimates. See here for the full ARC report.
Wednesday, 3 October 2012: See the location and view from the new lab at UWA, which will be ready to go by mid-December 2012.
Thursday, 23 August 2012: Josh will give his 'new academic staff' talk titled "By way of introduction ..." at UWA's Bayliss Building on 23-Aug, details here.
Wednesday, 25 July 2012: After an announcement by the Australian Research Council today, it's confirmed we'll be moving to UWA's School of Chemistry and Biochemistry in Perth to start a new lab in 2013! See the UWA press release here.
Friday, 15 June 2012: The Australian Society of Plant Scientists just announced Josh Mylne will be the 2012 recipient of the Peter Goldacre Award. The medal presentation and Goldacre plenary lecture will be on Tuesday 25 September at ComBio2012. UQ press release here.
Thursday, 5 July 2012: A 2-day workshop we've organised on non-model de novo transcriptome assembly starts today.
Monday, 18 June 2012: JBC paper on kalata-type cyclic peptides discovered in the common garden plant Petunia is on-line now via PubMed.
Tuesday, 5 June 2012: Our manuscript describing an interesting case of biosynthetic parallel evolution was accepted today by The Plant Cell, more details soon.
Wednesday, 31 May 2012: There will be an art show this Friday 18 May to co-incide with Fascination of Plants Day. It will be held at IMB from 10 am until 4 pm. See this 'link' for details.
Tuesday, 8 May 2012: We will be joined next week by Achala Jayasena of Sri Lanka who will pursue her PhD with support from an International Postgraduate Research Scholarship and a University of Queensland Centennial Scholarship. Achala did her Masters on Arabidopsis auxin and development with Lawrence Hobbie at Adelphi University in New York. She will start a project on a new class of seed peptides.
Wednesday, 11 April 2012: Today, local artist Sophie Munns has started a 3-week stint as an 'artist-in-residence' with us at IMB where she will gain inspiration for her 'homage to the seed' series of artwork as well as prepare an exhibition in time for the 2012 International Fascination of Plants Day on Friday 18 May.
Tuesday, 6 March 2012: On Sunday 25 March at 3 pm Josh will guest speak at the unveiling of a statue in Toowoomba where he will extol the virtues of sunflowers and their origins on the Darling Downs. The event honours Toowoomba, its educational institutions and the late Councillor Ian Orford and will take place at Lions Park on Tourist Road. See the Sunflower Tribute website for details or to make a donation. An article on the unveiling may be found here.
Tuesday, 22 February 2012: Our 'mini hot phenol' RNA extraction protocol has been put on-line. This arab-gen post described problems getting mRNA and sRNA from young Arabidopsis seedlings using Trizol. We responded on arab-gen having seen this before. We compared 'mini hot phenol' to Trizol in Fig 1 of this paper by Mathew Box et al.
Friday, 3 February 2012: On Monday 13 February, Josh will present results from a collaboration with the John Innes Centre at the XXII International Congress on Sexual Plant Reproduction in Melbourne "A living system to study chromatin dynamics of FLOWERING LOCUS C during vernalization".
Tuesday, 29 November 2011: UQ undergraduate Joseph O'Neill has been awarded a IMB summer scholarship to study drug mode of action using forward mutagenesis in the model plant Arabidopsis thaliana.
Monday, 1 October 2011: The Australian Research Council has awarded us a grant to study the biosynthesis of drug-like peptides in sunflower, which will improve our ability to manipulate plant systems to manufacture protein drugs.
Press and Media Archive
Below are links to past press releases and media highlights.
"Roundup Row: The row over the world's most popular weed-killer" ABC Landline with Philippa Courtney TV Interview, 5-Sep-2019
"The Layman's Podcast: Ep #9 - Dr Josh Mylne" You Tube, 5-Aug-2019
"The Layman's Podcast: Ep #9 - Dr Josh Mylne" Podbean, Aug-2019
"Proteins date back to the time of sabre toothed cats" UWA, Press Release, Mar-2018
"Antibiotic analogue puts researchers on path to ending herbicide drought" Chemistry World, Feb-2018
"Scientists reveal double life of sunflower enzyme" UWA, Press Release, Jan-2018
"Researchers from UWA awarded Fulbright scholarships" UWA, Press Release, Dec-2017
"Antimalarial drugs could provide future herbicides" by Stephen Luntz IFLScience.com
Radio: ABC Country Hour for Monday 3 July, 2017 ABC Country Hour, starts @09:20
"WA scientists use malaria chemicals to craft new herbicides" by Kit Mochan ABC Rural News, Jun-2017
"Antimalarial drugs offer a smorgasbord of new herbicides" UWA, Press Release, Jun-2017
UWA video for World Malaria Day 2017 UWA FaceBook, 25-Apr-2017
"Malaria Curing Chemicals Kill Weeds Too" Leading Agriculture, 5-Apr-2017
"Chemicals that cure malaria can kill weeds too" UWA, Press Release, 3-Apr-2017
"Proteins hiding in proteins take an evolutionary shortcut" UWA, Press Release, 27-Mar-2017
"Protein doppelgangers are long-lost cousins" UWA, Press Release, 15-Mar-2017
"Common antibiotic inspires hunt for new herbicide" UWA, Press Release, Feb-2016
"Sunflower enzyme both cuts & glues proteins" Asian Scientist, May-2015
"Sunflowers: source of stability in proteins" Aust. Life Scientist, May-2015
"Sunflower protein 'scissors' provide sunny news for medicine" UWA, Press Release, May-2015
"Ancient buried treasure found in daisy seeds" UWA, Press Release, Mar-2014
Interview on evolution 42evolution.org interview, 26-Sep-2014
"Moving genes have scientists seeing spots" UWA, Press Release, Sep-2013
"Flowers a cancer hope" The West Australian, Jun-2013
"Prickly questions" by Graeme O’Neill Aust. Life Scientist, Sep-2012
"Healing gac fruit" Good Fruit and Vegetables
"Scientists from the University of Queensland are trying to find ways of creating cheaper cancer treatments" by Jonathon Howard Westside News, 8 Aug 2012
"Future Fellows to ignite passion for research" UWA, Press Release, Jul-2012
"Seed genes could provide basis for affordable therapeutic drugs" UQ, Press Release, Jul-2012
"UQ Scientist awarded for cracking sunflower's secret protein codes" Westside News, 17 July 2012
"Sunflower seed drug discovery earns national award" UQ, Press Release, Jun-2012
"Pharmland" by Becky McCall COSMOS magazine, Feature Article
"Could we grow drugs using sunflowers?" Science in Public, Media Release
"Co-opting nature to make cheaper drugs" by Tim Dean Aust. Life Scientist, Mar-2012
"Flower Power" Channel 10 News
"Could we grow drugs using sunflowers?" XVIII IBC News, Jul-2011
"Cancer drug found hiding in sunflower seed protein" UQ, Press Release, Mar-2011
"Recognizing Our Authors: ASPB Top Authors Australasia" ASPB Top Authors 2004-2008